Sentence examples for mutagenesis method we from inspiring English sources

The site-directed mutagenesis method we present here circumvents the use of primer pairs in the same PCR.

Using reverse engineering and site directed mutagenesis methods, we were able to confirm that the amino acid substitution of the Asp285 is responsible for the increased aldehyde reductase activities by utilizing the additional cofactor NADPH.

We developed a rapid mutagenesis method based on a modification of the QuikChange® system (Stratagene) to systemically replace endogenous gene sequences with a unique similar size sequence tag.

We report here the development of a random mutagenesis method for directed evolution of Streptomyces lividans acetyl xylan esterase (AxeA), which we previously showed is able to deacetylate chitinous substrate, in order to obtain chitooligosaccharides with well-defined structural properties.

We designed a bridge-mediated mutagenesis method to generate Foxp3 mutants with complete deletion of each of the domains.

Here, we describe a general transposon mutagenesis method that allows for removal of up to five consecutive in-frame codons from a random position of a target protein.

In this report, we have outlined an RNA mutagenesis method that was specifically developed to provide an improved approach for the in vitro evolution of proteins, in particular, as an effective tool that can be coupled to ribosome display.

Here, we present a rapid efficient gene splicing and multi-sited mutagenesis method that introduces mutations at two distant sites via sequential connection of DNA fragments by one-step overlap extension polymerase chain reaction (OE PCR).

We constructed deletion cassettes for use with the λ Red-mediated site-directed mutagenesis method to delete either guaBA, htrA or clyA from wildtype S. Typhi Ty2.

Mutagen™: A Random Mutagenesis Method Providing a Complementary Diversity Generated by Human Error-Prone DNA Polymerases / Hakim Kharrat -- 27.

For the random mutagenesis method, there were many white colored colonies generated indicating a high rate of negative mutation.