Sentence examples for mutagenesis generating a from inspiring English sources

In the current study, we examined the effect of the CORE-GS insertion on the efficiency of SSG mutagenesis, generating a "hybrid" method between delitto perfetto and SSG mutagenesis that we term "gsSSG mutagenesis".

Although we cannot rule out the latter possibility, it may be a less likely explanation, because a different protein, rGH (rat growth hormone), which is normally a non-glycosylated secretory protein, can be transported to the cell surface when it is forced to be N-glycosylated by mutagenesis generating a glycosylation consensus sequence (Asn-Xaa-Ser/Thr) [ 35].

Further structure-guided mutagenesis generated a quadruple mutant (Y50F/N110S/I212T/L264V), whose KM for isobutyraldehyde is ∼17-fold lower and catalytic efficiency (kcat/KM) is ∼160-fold higher than wild-type LlAdhA.

ENU mutagenesis generated a ti/tv ratio of 1.2, slightly higher than observed in the spontaneous mutations.

To determine the importance of conserved residues of phiC31 integrase during recombination, a series of 19 conserved residues were targeted by site-directed mutagenesis, generating 17 mutants (Table 1).

The C616Y amino acid change was introduced by site directed mutagenesis, generating pUAS-FLAG-Fbxl7C616Y pUAS-FLAG-Fbxl7C616Y pUAS-FLAG-Fbxl7C616Y

This mutagenesis generates additional unmethylated recognition sequences for some restriction modification systems with a methyltransferase protecting DNA with an m6A methylation.

Though cancer has long been considered a monoclonal process, recent studies show that ongoing mutagenesis generates subclonal populations whose numbers wax and wane depending on the variant's relative evolutionary fitness [ 1- 5].

Site-directed mutagenesis generated the fully humanized product and four intermediate scFvs, which were analyzed for protein expression and antigen binding.

Successive rounds of mutagenesis generated active GFP variants containing, three, two, and zero Phe residues.

We used the experimental data (chemical shift mapping and mutagenesis) to generate a model of the interaction docking peptPP2 on the known structure of NPHP1-SH3 using the HADDOCK strategy [18].