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Mutation detective PCR revealed the mutagenesis frequency of the individual genes to be between 72.9% and 100%.
The mutation detective PCR revealed that the mutagenesis frequency of the individual genes was between 72.9 and 100% (Fig. 4C).
To calculate the mutagenesis frequency of the new strategy, several colonies from each experiment were picked and sequenced (Fig. 2).
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Exposure to histone deacetylase inhibitors prior to HSV1m5/Trex2 treatment increased mutagenesis frequencies of latent HSV genomes another two- to fivefold, suggesting that chromatin modification may be a useful adjunct to gene-targeting approaches.
The percentage of digested amplicons provides an estimate of the somatic mutagenesis frequency.
Progeny from line 10 and line 15 (30% mutagenesis frequency) were screened for the presence of inherited mutations at the NATA2b target site.
Different lines of Arabidopsis with integrated TALEN constructs varied considerably in somatic mutagenesis frequencies, likely because of variable expression of the TALEN transgenes.
It is also possible that chromatin structure and packing of DNA in the environs of a gene may play a role in its mutagenesis frequency.
At such mutagenesis frequencies the number of induced mutations is orders of magnitude above the accumulation of spontaneous mutations, yet is not too large as to preclude organismal viability [1].
However, knockdowns of BER components showed that single knockouts provided minor, if any, effect on the mutagenesis frequency.
In these cases, we reasoned the percentage of uncut DNA by these restriction enzymes should approximate the ZFN-mediated mutagenesis frequency.
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