Sentence examples for mutagenesis frequencies from inspiring English sources

Coexpression of the 3′-exonuclease Trex2 with HEs increased HE-mediated mutagenesis frequencies up to sixfold.

Exposure to histone deacetylase inhibitors prior to HSV1m5/Trex2 treatment increased mutagenesis frequencies of latent HSV genomes another two- to fivefold, suggesting that chromatin modification may be a useful adjunct to gene-targeting approaches.

For common mutagenesis frequencies, the last term is exceedingly small and can be ignored without significant loss of accuracy.

At such mutagenesis frequencies the number of induced mutations is orders of magnitude above the accumulation of spontaneous mutations, yet is not too large as to preclude organismal viability [1].

Mutagenesis frequencies in lines 1, 7, and 11 were 12%, 42%, and 33%, respectively.

Although there are currently three single PCR tube strategies available, ours is simpler and yields mutagenesis frequencies approaching 100%.

On the target-1, only 2 mutants were identified from 96 independent lines using hSpCas9 (2.1% mutated), compared to the 15.6% mutagenesis frequency using pSpCas9 (Figure 1b).

The significant different on the mutagenesis frequency at the target-1 (P < 0.01, χ2 test) might be related to the higher level of Cas9 protein accumulation in the pSpCas9 transgenic plants than in the hSpCas9 transgenic plants (Additional file 3: Figure S2), similar to previous results that were obtained by transient assays (Li et al. [2013b]).

It is also possible that chromatin structure and packing of DNA in the environs of a gene may play a role in its mutagenesis frequency.

Nonetheless, in many instances, assuming an average mutagenesis frequency can lead to useful estimates regarding screen progress, and deviations from these estimates can often hint at unique features of a gene, such as unusually large or small size [1], [12], [13].

The average mutagenesis frequency obtained with this strategy is about 50%.