Sentence examples for mutagenesis creating a from inspiring English sources

It involves subjecting a gene to iterative rounds of mutagenesis (creating a molecular library with sufficient diversity for the altered function), selection (expressing the variants and isolating members with the desired function), and amplification (generating a template for the next round).

The Q73L substitution mutation was introduced into a CIN4 expression plasmid (pJM0230) by site-directed mutagenesis, creating a cin4-Q73L explasmidn pJM0231 (pJM0231).

Extensively used, mutagenesis creates a large and discrete spectrum of lesions that are subsequently detected by forward or reverse genetic approaches.

Additionally, unintended effects of the genetic modification itself cannot be excluded, as direct or indirect consequences of insertional mutagenesis, creating possible unintended metabolic effects.

The frameshift between the PK and the GFP was corrected by site-directed mutagenesis, creating PK-GFP.

In order to proof this hypothesis by experimental data, the CUG, the AUG and both codon sequences together were changed by site directed mutagenesis creating three new expression constructs named PEG10-RF1a/2mutATG-His PEG10-RF1a/2mutATG-His PEG10-RF1a/2mutATG-HisG/CTG-His.

The first three nucleotides of the miRNA binding site in the 3'UTR were mutated by site directed mutagenesis, creating the 3'UTR-mut-pGL3 vector.

Here, site-directed mutagenesis created Elk-1 mRNA mutated at a single site.

Furthermore, we used site-directed mutagenesis to create a variant of the LDLRAP1 construct in which the MIR168a target site was mutated while leaving the LDLRAP1 amino-acid sequence intact (Figure 3I).

Through structure-guided mutagenesis, we created a mutant ligase capable of recognizing a 7-hydroxycoumarin substrate and catalyzing its covalent conjugation to a transposable 13-amino acid peptide called LAP (LplA Acceptor Peptide).

Sandstrom used acridine orange mutagenesis to create a mutant library which was screened for "rough" colonies on solid media; the "rough" colonies were designated as lacking capsule.