Sentence examples for mutagenesis could be from inspiring English sources
In this study, we sought to determine whether retroviral insertional mutagenesis could be used to isolate cell mutants, in which the transcriptional activation of a receptor gene has occurred.
Importantly, insertional mutagenesis could be used to generate pools of randomly genetically-altered cells or organisms for forward genetics applications.
Site-directed mutagenesis could be used to establish which positions within this putative 7-bp overlap region of attB sites are critical for the integration of SGI1.
Furthermore, genome-wide mutagenesis could be performed to identify genes that promote or suppress liver tumorigenesis.
Additional mutagenesis could be used to define this upstream cleavage site and to determine whether proteolysis at one site affects sensitivity to cleavage elsewhere.
The rapid evolution and large mutation frequencies of norovirus replicating in immunocompromised patients (Bull et al., 2012) suggest that antiviral mutagenesis could be an effective approach.
Both phylogenetic and rational site-directed mutagenesis approaches could be keys to identifying specific amino acids that are responsible for the differences in activity between the proteins.
As ENU-induced DNA damage is normally recognized by the mismatch repair (MMR) system, we hypothesized that the effectiveness of the target-selected mutagenesis approach could be improved by using a MMR-deficient genetic background.
Under these very light mutagenesis conditions, it could be predicted that the Ctf mutants identified would most likely be the result of mutations in single genes, thus simplifying follow-up experiments.
It is probably fair to say that even with the improved efficiency of piggyBac insertion demonstrated by Fonager et al. [ 4], this system still falls short of a 'saturating' mutagenesis procedure that could be easily used for screens.
Such studies naturally led to the general consensus that once a putative site for the formation of a Tyr radical is suppressed (by site-directed mutagenesis) the radical could be formed on other Tyr(s), with the underlying interpretation that the formation of Tyr radicals in CcP may be a rather random event, with no specific electron transfer pathway and/or catalytic function involved.
