Sentence examples for mutagenesis consisted of from inspiring English sources

PCR conditions for mutagenesis consisted of an initial 2 min denaturation step at 95 °C followed by 19 cycles of 50 s at 95 °C, 50 s at 60 °C and 13 min at 68 °C, and a final 7 min elongation step at 68 °C.

A knockout vector was obtained during the mutagenesis, consisting of the REMI vector flanked by genomic regions comprising fspA gene (Fig. S1).

A plasmid containing dominant negative human HSF1 (hHSF1-DN; with deletion of amino acids 453 523; [ 19]) was constructed by PCR-mediated site-directed mutagenesis consisting of two-step PCR, using two overlapping internal primers at the mutagenic site and two outer general primers each flanked by HindIII site.

A typical mutagenesis experiment consists of overnight growth of the strain under study followed by the actual mutagenic treatment and a recovery step.

Therefore, it is advised to change the type of mutagen in a mutagenesis program consisting of multiple rounds, in order to sample as many different types of genetic changes as possible (Rowlands, 1984).

Mutant constructs were generated by PCR-based mutagenesis: C. elegans PCH-2 ΔNTD consisted of residues 100 424, and the 'Pore loop AG' mutant replaced residues 218 227 with the protein sequence 'AGAAGAAAGA'AGAAGAAAGA

For the industrial production of β lactams, the filamentous fungus Penicillium chrysogenum has been subjected to an intense classical strain improvement (CSI) program that consisted of repeating rounds of mutagenesis and selection.

Previous mutagenesis of gp64 in the same region did not inactivate gp64 expression but consisted of the addition of residues not their change [ 36].

The first stage consisted of a set of two PCR reactions: one from an upstream flanking primer (F1) to the negative-sense mutagenesis primer (R1) and the other from a downstream flanking primer (F2) to the positive-sense mutagenesis primer (R2).

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