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Site-direct mutagenesis was performed according to the manufacturer instructions (Stratagene) verifying the correct mutagenesis by sequencing.
After confirming the success of NDT mutagenesis by sequencing of random clones, the pglB plasmid library was re-transformed in the expression strain E. coli CLM24 (pGVXN345, pGVXN345) and screened with the DWP-ELISA system.
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Successful cloning and mutagenesis was verified by sequencing.
Mutagenesis was verified by sequencing.
Mutagenesis was validated by sequencing.
The correct mutagenesis was confirmed by sequencing.
The constitutively active forms (Q68L) were generated by point mutagenesis and verified by sequencing Rab39B.
The K510R mutation was generated by site directed mutagenesis and verified by sequencing.
SPRED1 mutant p.Trp31Cys was generated by PCR-directed mutagenesis and verified by sequencing.
Success of mutagenesis was confirmed by sequencing with gene-specific primers.
K614E and V443I mutations in hOCA2 were made using site-directed mutagenesis and verified by sequencing.
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