Exact(7)
This last issue is important, in order to try to avoid as much as possible an altered emergence frequency of mucoid variants due to differential selection instead of differential mutagenesis between mucA alleles.
To construct the insertion mutants, the Gly (G), Gly-Gly-Gly-Gly-Gly (G5), Gly-Gly-Gly-Gly-Gly-Gly (G6), His-His-His-His-His-His (H6) and Asp-Asp-Asp-Asp-Lys (D4K) sequences were each inserted by PCR mutagenesis between the positions Pro113 and Thr114 of the furin sequence.
In order to determine other properties in the sequence of mucA, which might lead to differential mutagenesis between domains, we further sought for all the possible positions at which premature stop codons could be generated by C-to-T transitions (which is the other large group of mutations observed in mucA).
In addition, we did not observe significant efficiency differences in the tissue-specific mutagenesis between two independent gRNA transgenic lines from the same transgenic gRNA.
Comparative mutagenesis between glyoxal and methyglyoxal in African green monkey kidney (COS-7) cells using the supF reporter gene showed dramatically different mutagenic spectra.
Early crystallographic evidence suggested that in the monomeric form of PA, this loop becomes disordered at low pH, and biochemical experiments have also suggested that interactions with this loop dictate the pH threshold for pore formation, that the loss of contacts (via mutagenesis) between PA and this loop increases the pH requisite for pore formation.
Similar(53)
Regarding the different level of mutagenesis observed between G5SSR426 and C6SSR431, it should be considered that not only their length, but also their orientations respect to the replication fork may influence the mutagenesis outcome.
However, mutagenesis cannot distinguish between protein-based and ligand-based mechanisms, nor can this site explain the complex modulation by propofol.
Importantly, we document for a strong positive correlation between mutagenesis efficiency and sgRNA GC content of the six protospacer-adjacent motif-proximal nucleotides (PAMPNs).
These 21 genomes have yielded further evidence of complex relationships between mutagenesis and transcription.
We have now emphasised this link between mutagenesis and decreased fitness and specific infectivity in the text.
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