Sentence examples for mutagenesis based on from inspiring English sources

Rational mutagenesis based on x-ray structures and sequence alignment, as well as random methods such as directed evolution, have been utilized.

Here we report on construction of a riboflavin overproducing strain of C. famata using a combination of random mutagenesis based on the selection of mutants resistant to different antimetabolites as well as rational approaches of metabolic engineering.

Further mutagenesis based on the Q88A/R275A variant resulted in 5.2 times improvement compared to the wild-type p-nitrophenyl cellobioside hydrolyzing activity, which is the best improvement obtained so far for an engineered xylanase.

CFP-Kvβ2K235E was made by Quickchange mutagenesis based on CFP-Kvβ2.

For example, KaiCTST is for the wild-type KaiCT426/S431/T432 (i.e. KaiCWT), KaiCaST for KaiCT426A/S431/T432, KaiCTae for KaiCT426/S431A/T432E, etc. Mutation of KaiC at 426, 431, and 432 was performed by site-directed mutagenesis based on template plasmids harboring either wild-type kaiABC or kaiC DNA.

Chief among these is conditional mutagenesis based on the use of Cre- loxP site-specific recombination (Branda and Dymecki, 2004).

Recent methodology development in directed evolution of stereoselective enzymes has shown that various mutagenesis strategies based on saturation mutagenesis at sites lining the binding pocket enable the generation of small and smart mutant libraries requiring minimal screening.

To create a PDR16 allele encoding a protein defective in PI binding, plasmid YEplac181- PDR16 was modified using in vitro site-directed mutagenesis protocol based on Agilent Site-Directed Mutagenesis Kit (Agilent, USA) with minor modifications.

When performed under an error-prone condition with Taq DNA polymerase, PPCP allows one-step construction of mutagenesis libraries based on in situ error-prone PCR so that random mutations are introduced into the target gene without altering the expression vector plasmid.

The best mutant AcM1 was identified from random mutagenesis libraries based on its growth performance.

Genetic tools and mutagenesis strategies based on transposable elements are currently under development with a vision to link primary DNA sequence information to gene functions in vertebrate models.