Sentence examples for mutagenesis at position from inspiring English sources
Because ATP is an allosteric activator of the epimerase and the binding of the nucleotide is crucial for its catalytic properties, saturation mutagenesis at position K160 was performed to identify the most compatible amino acid exchanges.
To create biocatalysts suitable for the biosynthesis of indirubin (a drug for chronic myelogenous leukemia and a novel promising anticancer agent), cytochrome P450 BM-3 was engineered by site-directed saturation mutagenesis at position D168 to alter its hydroxylation regioselectivity towards indole.
Mutagenesis at position 143 was performed using the QuikChange Site-directed Mutagenesis kit (Stratagene) according to the manufacturer's instructions.
Site directed mutagenesis at position Cys12 in PsTRXh1, and at position Cys56 in PsTRXf were carried out by using the Quick-Change-Site-Directed Mutagenesis kit (Stratagene, USA).
The construct having EhCaBP1 gene in pET 3(c) vector was used as a template and a stop codon was introduced by site directed mutagenesis at position 199.
A stop codon was introduced by Phusion mutagenesis at position 110 for the experiment in Figure 7D.
We decided to perform serial deletions by introducing STOP codons by site-directed mutagenesis at positions E679X, E769X, Q863X and E1020X.
Specifically, alanine substitution mutagenesis at positions K133A and R134A of VP2 and of the VP2 136-138A clone was carried out to further characterize the nuclear localization motif and the results were summarized in Table 1.
Surprisingly, the specificity of the evolved aldolase from the third generation of DNA shuffling (H26Y/D104G/V121A/P256L) was not further improved through saturation mutagenesis at positions 26, 104, 121, and 256.
16 For the smFRET measurements, donor (D; Alexa Fluor 488) and acceptor (A; Alexa Fluor 594) FRET probes were attached to A30P α-synuclein through the reaction of maleimide dyes with cysteine residues incorporated by site-directed mutagenesis at positions 7 and 84 (see the Supporting Information).
Saturation-mutagenesis at position TmoA I100 resulted in the finding of novel HTyr-producing variants I100A, I100S and I100G.
