Sentence examples for mutagenesis and sequencing from inspiring English sources
In vitro transposon mutagenesis and sequencing of the insert DNA in this clone revealed an ORF of 1890 bp encoding a protein with 630 amino acids which showed significant sequence homology to the peptidase S8 and S53 subtilisin kexin sedolisin of Shewanella sp. This ORF was cloned in pET30b and expressed in E. coli BL21 (DE3).
Oligonucleotides for mutagenesis and sequencing were synthesized by Operon Technologies, Inc (Huntsville AL).
We next performed bisulfite mutagenesis and sequencing of the Peg3 gDMR to more thoroughly examine individual gDMR cytosine methylation status.
Enzymes used for DNA manipulation were from Promega (Madison, WI) and DNA oligonucleotides for mutagenesis and sequencing were from Integrated DNA Technologies Coralville, IAA).
Pairs of primer used for mir-8 mutagenesis and sequencing.
Cloning, mutagenesis, and sequencing were performed by Top Gene Technologies (Pointe-Claire, QC).
Genome editing (GE) tools ensure targeted mutagenesis and sequence-specific modification in plants using a wide resource of customized endonucleases; namely, zinc-finger nucleases (ZFNs), and transcription activator-like effector nucleases (TALENs), and the CRISPR (clustered regularly interspaced short palindromic repeats)/Cas (CRISPR-associated protein) system.
Oligonucleotides used for plasmid construction, site-directed mutagenesis and sequence confirmation are listed in Additional file 1: Table S2.
D620N and P316S mutations were introduced by PCR-mediated site-directed mutandnesequencedequenced to confirm their integrity.
This knob structure is bound close to rRNA and rp17e in the established 40S structures, but mutagenesis and sequence deletion studies indicate that it is not actually essential for binding of RACK1 to the ribosome, at least in the case of yeast [ 79].
Mutants were made using polymerase chain reaction mutagenesis and DNA sequencing was performed by The Sequencing Service, University of Dundee (www.dnaseq.co.uk).
