Since the viral genome can be carried as an episome in bacterial cells, prokaryotic genetic techniques can be employed for manipulation of the viral genome without the need for typical selective mechanisms required for mutagenesis and selection in eukaryotic cells.
Arnold developed iterative methods that mimicked the random mutagenesis and selection found in nature and developed screening techniques to select mutations that addressed targeted properties.
Comparable cefotaxime resistant variants were selected relatively quickly (only two rounds of mutagenesis and selection) and with no silent or other superfluous mutations, an approach that was accomplished through what we have termed a "minimal mutational pathway".
An industrially important cellulolytic strain of T. reesei, Rut-C30, was selected for improved cellulase production following multiple rounds of mutagenesis and selection.
After four generations of mutagenesis and selection, we obtained enzyme variants with new specificity for GATT.
The creation of genetic diversity through mutagenesis and selection is essential for organisms to adapt to the changing environments.
We analyzed a mathematical model of in vitro evolution conducted by repetition of mutagenesis and selection processes.
The fact that codons 248 and 273 are often the targets of G to T transversions in lung cancers is a perfect illustration of the respective roles of mutagenesis and selection in shaping the mutation spectrum of p53.
Using an AMA1-specific IgNAR single-variable-domain antibody, we performed targeted mutagenesis and selection against AMA1 from three P. falciparum strains.
Currently, the mutant of the flavinogenic yeast Candida famata dep8 isolated by classic mutagenesis and selection is used for industrial riboflavin production.
Two rounds of random PCR mutagenesis and selection experiments increased the congerin II thermostability to a level comparative to its naturally thermostable isoform, congerin I.
