High efficiency of its transposase, recently enhanced more than 100-fold by directed mutagenesis [ 24], and random chromosomal insertion make the SB system a method of choice for many applications [ 25].
Structure-based design [1], [2], sequence alignment approaches [3], [4], and random mutagenesis [5], [6] are all used to stabilize proteins by mutagenesis, but problems still exist, especially in finding simple and general techniques.
A combination of rational design and random mutagenesis was used to achieve the desired catalytic migration.
Here, we employ domain substitution and random mutagenesis to recapitulate NRPS evolution, focusing on PCP domains.
The combined approaches of rational design and random mutagenesis were applied to generate a sesquiterpene synthase with an altered activity.
Efforts to generate new specificities by structure-guided design and random mutagenesis have been unsuccessful so far.
The mechanism of organic solvent tolerance has been investigated by Ogino et al. [34] in a Pseudomonas aeruginosa PST-01 protease by site-directed and random mutagenesis.
Moreover, FACS has recently prompted several approaches for the effective improvement of microalgal strains by means of genetic engineering, serial selection and random mutagenesis.
In this work, further understanding of the significant and essential interactions between the ligand and the receptor was deciphered, through a combination of rational and random mutagenesis.
Directed and random mutagenesis, the classical methods of enzymatic engineering, have proved to be efficient in some cases, but are quite tricky.
