Sentence examples for mutagenesis and expression from inspiring English sources
Previous in vitro mutagenesis and expression of these modifications (G137D, in the oxyanion hole and W251L, in the acyl pocket) have confirmed their functional significance.
The tsA201 cells were transfected with WT or mutant rNav1.4 cDNA contained in the mutagenesis and expression vector pAlter-Max (Promega) (10 ug) [73] along with human β1-subunit (10 ug) cDNA utilizing the calcium phosphate method and grown to 40 50% confluence on 100 mm plates [72], [74].
Mutagenesis and expression constructs.
The cloning, mutagenesis, and expression of the N-terminal domain of human cardiac TnC (C35S, C84S, residues 1−89) have been reported previously (14).
Site-directed mutagenesis and expression of mutant receptor in vitro Point mutations were introduced using the QuikChangeXL Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA, USA) according to the manufacturer's instructions.
These results indicate that TFBS gain or loss events at NDRs may have a higher probability of causing expression differences than those at NRs. Twelve genes that experienced TFBS gain or loss events at both NDRs and NRs were selected for site-directed mutagenesis and expression analysis.
Mutagenesis, cloning and expression of this synthetic gene fragment, defined as FMP003 has been described elsewhere [25].
Our results also suggest that the integration of mutagenesis data and expression network analysis may help to unravel key events in other cancers which disrupt complex developmental programmes, for which murine models are available.
The recent development of effective genetic tools for Rhodococcus, such as unmarked gene deletion, transposon-based mutagenesis, and gene expression systems, now allows the construction of biocatalysts with desirable properties for industrial purposes.
Even today, in spite of the fact that many eukaryotic expression systems are well established, various factors, such as cost-effectiveness, insertional mutagenesis and transient expression, still need to be resolved for these systems.
Here we present a genetic screen that combines retroviral mutagenesis and cDNA expression cloning to reveal proteins involved in the extracellular regulation of latent TGFβ activation.
