were focussed on 2,5-DKG reductase optimization by site-directed mutagenesis and the kinetic characterisation of the obtained mutants after expression in E. coli, rather than the optimization of expression yields (Banta et al. 2002a, b, Powers 1996, Sanli et al. 2004, Banta and Anderson 2002 ).
Site-directed mutagenesis and a comparative characterisation of the kinetic parameters, pH dependency of activity and thermal stability of mutant and wild-type enzymes have been used in association with crystallographic analysis to delineate the functions of several active site residues in a novel glycoside hydrolase family 8 xylanase.
Site-directed mutagenesis has been central to the characterisation of cellulases, ranging from the identification and characterisation of putative catalytic and binding residues, the trapping of enzyme-substrate complexes by crystallography through to the construction of new and improved biocatalysts including 'glycosynthases'glycosynthases
Plotting and characterisation are tendentious.
For Bay, trifles such as plot and characterisation are tiresome.
However, the real artistry here is in the costumes, production design and characterisation.
Serial DWI monitoring is of value in detection and characterisation.
The critical response was negative, directed mainly at plot holes and characterisation.
Smith stated, "clunky plotting and characterisation mean it has 'telemovie' written all over it".
DNA and RNA preparation and characterisation, sequencing procedures and PCR methods were described previously [14], [15].
