Site-directed mutagenesis analysis have shown that Lys11 and Lys90 are vital for the bioactivity of TNF-α[30, 31].
Open image in new window Figure 4 Sequence alignment of BubR1 and mutagenesis analysis of the B56γ1-BubR1 B56γ1-BubR1
To validate the crystallographic observations and evaluate the significance of individual interaction, we performed mutagenesis analysis on both sides of the PP2A B56 and BubR1 interface.
Site-specific mutagenesis analysis of C. necator PHA synthase had shown that the conserved cystein-319, aspartate-480 and histidine-508 of the class I synthase are required for enzyme activity.
Mutagenesis analysis showed that phosphorylation at serine 174 is required for Atg31 to carry out its function, while the other phosphorylation sites have no function in the regulation of autophagy.
We conclude that our approach, based on DSC and site-directed mutagenesis analysis of chimeric fusion proteins, may serve as a suitable tool to analyse the energetics of weak biomolecular interactions such as those involving SH3 domains.
In order to clarify the genotoxicity of bitumen fumes, we designed a study by using mice carrying a reporter gene for mutagenesis analysis and exposed by nose-only to a constant and reproducible aerosol of bitumen fumes.
Although improvements in vector design clearly lowered the risk of this insertional mutagenesis, analysis of potential genotoxicity and the consequences of vector integration remain important parameters for basic and translational research and most importantly for the clinic.
Mutagenesis analysis indicated that hydrophobic interaction was dominant for the recognition of crystalline chitin and that the acidic residues were responsible for a higher substrate specificity of ChBD2 for chitin compared with that of cellulose.
We also performed in silico mutagenesis analysis to characterize the effects of oncogenic mutations on the negative regulators and to observe the cellular outcome (whether there is/is not inflammation).
Particularly notably, the P-loop from each TD contribute 6 7 residues to form a geometrically constricted region at the extracellular side of the PD, which is mainly responsible for ion selection according to mutagenesis analysis (Favre et al., 1996; Schlief et al., 1996; Sun et al., 1997) and is therefore named as the selectivity filter (SF).
