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Mussel sampling was done bimonthly over two separate periods of 11 months each.
We are most grateful to Breea Govenar and Brandi Cron for onboard assistance with mussel sampling and tissue preparation after the Lucky Strike dives.
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These data showed a clear temporal pattern in transcriptomic profiles of mussels sampled over an annual cycle.
With these goals, we examined mitochondrial COI and portions of five nuclear genes from mussels sampled along a 5600-km range from 13°N to 38°S latitude.
Total RNA was purified from two hemolymph pools (N = 10) per time point, and from paired saline-injected control mussels sampled at 3 and 48 h (N = 40, one unique reference pool).
Considering the mussels first, the main difference between the BOLD mussels and the mussels sampled in the other Northeast coast estuaries is that while the other mussels have a Hg isotopic composition consistent with a mixture of IHg and MMHg from the sediment, this pattern was observed only for ΔHg, but not δHg in the BOLD mussels.
The analytical procedure was applied to mussel samples from Galicia Spainn).
The optimized method was applied to the extraction of real mussel samples from two Galician Rías.
This method was successfully applied to the analysis of wild mussel samples coming from two Galician Rías.
The proposed method was finally applied to 16 mussel samples (Mytilus galloprovincialis) from Ría de Arousa estuary (Galicia, northwest Spain).
Copper was extracted on-line from solid mussel samples by a simple and rapid continuous ultrasound-assisted extraction system (CUES).
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