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Mussel condition index and phosphorus concentrations were correlated and increased west-to-east within the U.S. nearshore.
We used a non-parametric Kruskal Wallis to compare zebra mussel condition factor and tissue C∶N∶P stoichiometry among months.
Zebra mussel condition factor was weakly related to the their own tissue N∶P and C∶P ratios, although the relation with the later ratio was not significant.
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After an acclimatizing period of 6 days, mussels were divided in groups of 5 vessels per 30 individuals each (150 mussels per condition) and further used as control reference or subjected to semi-static exposure to chemicals.
Thus small mussels with good condition index and high growth rate require food with high P content to maintain optimal growth and reproduction and are therefore, more likely to suffer from reduced food quality.
Both clearance rate values and immune parameters analysis indicated that the health condition of mussels kept in 50 ml tubes for 24 h or 48 h was not altered compared to controls, while most parameters were depressed after 72 h.
In their native range, infested mussels can have reduced condition index, shell growth and reduce shell strength [16], [31].
Mussels (40 individuals per condition) were exposed for 4 days to nickel and Chlorpyrifos respectively in the range of 0.01-15 mg/L and 0.1-10 0.1-10
For toxicokinetics assessment, mussels (30 individuals per condition) were exposed for 4 days to nickel or Chlorpyrifos at the nominal EC50 for effects on LMS and to NiEC25+CHPEC25 in the case of the mixture.
Linear regression was also applied to analyze the relationships between mussels size (shell length) against tissue C∶P and N∶P ratios and condition of zebra mussels.
We proposed that multiple biomarkers may be employed as an integrated diagnostic tool for monitoring the health condition of the mussels.
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