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After each physical trituration, the muscles were transferred in a new well, to get rid of collagen wisps and hyper-contracted fibers.
Muscles were transferred to PBS.
After a 30-minute preincubation period, muscles were transferred to a fresh medium of similar composition but containing 0.5 mM C-Phe (Amersham Corporation, now part of GE Healthcare, Little Chalfont, Buckinghamshire, UK) to measure the rate of protein synthesis.
After removal from the animal, muscles were transferred into Krebs solution (118 m M NaCl, 4.7 m M KCl, 2.5 m M CaCl2, 1.2 m M MgSO4, 1 m M KH2PO4, 25 m M NaHCO3, and 11 m M glucose) chilled to 4°Celsius and perfused with 95%O2/5%% CO2 (pH 7.4).
Muscles were transferred to 2 ml KRB containing 1 mM 2-deoxy-D-[1,2-H(N ]glucose (3 μCi) and 7 mM D-[1-C]mannitol (0.9 μCi) and incubated for an additional 10 min with or without insulin at 30°C.
Similar(55)
This suggests that perhaps one half of the lactate transported out of fast muscles is transferred across the T tubular membranes in addition to the lactate released via the surface membranes.
Five grams of minced flying fish muscle was transferred to a 50-mL centrifuge tube and homogenized (Omni International Waterbury) with 20 mL of 6 % TCA for 3 min.
Following digestion, the muscle was transferred in plating medium (DMEM low-glucose, 10% HS, 1% penicillin-streptomycin, 0.5% chicken embryo extract, GIBCO-Invitrogen) and gently triturated with a wide-bore pipette to release single myofibers.
One half of the muscle was transferred to a microfuge tube and homogenized by hand in the lysis/binding solution supplied with the kit using a plastic pestle.
Left ventricular papillary muscle was transferred from resected mitral valve to physiological saline and liquid nitrogen and then moved to −80°C refrigerator for storage.
Following digestion, the muscle was transferred to 100 mm × 15 mm plastic petri dishes containing 10 ml of plating media (10% horse serum in DMEM) using fire-polished-tip Pasteur pipettes.
More suggestions(15)
muscles were obtained
muscles were found
muscles were fixed
muscles were immersed
muscles were determined
muscles were subjected
muscles were isolated
muscles were observed
muscles were measured
muscles were kept
muscles were treated
muscles were homogenised
muscles were incubated
muscles were chosen
muscles were weighed
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