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Study Design: Detailed dissections of the peripheral innervation of the iliococcygeal, pubococcygeal, puborectal, and coccygeal muscles were performed in 12 fresh-frozen female cadavers (aged, 32-100 years) with the use of transabdominal, gluteal, and perineal approaches.
All stimulations of the control and operated tibial nerves and electroneurographic (ENG) recordings of the compound muscle action potentials (CMAP) in the gastrocnemius muscles were performed, using a Vicking Quest electrodiagnostic device (Nicolet Viking Quest IV, Nicolet EBE GmbH, Kleinostheim, Germany).
Intramuscular pressure measurements of the lumbar back muscles were performed in various positions.
Open muscle biopsies (from left quadriceps or deltoid muscles) were performed, oriented and frozen according to standard procedures [ 53].
Weekly vaccinations into both anterior tibial muscles were performed thrice with 100 μg DNA per leg in a volume of 50 μl normal saline.
All electrophysiology (Fig. 3) was performed on ventral longitudinal muscle 6. whole-cell patch clamp measurements from embryonic muscles were performed as previously described [ 41, 43].
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Then proteomic profiling of skeletal muscles was performed.
Intramuscular microdialysis into masseter muscles was performed to sample serotonin (5-HT), glutamate, lactate, pyruvate, glucose and glycerol.
An en bloc resection of the tumor with a part of those muscles was performed (Fig. 2d).
Clinical assessment of temporomandibular joints and masticatory muscles was performed by one experienced and self-trained examiner according to the RDC/TMD recommendations [27, 28].
To prove this hypothesis, retrograde staining from mandibular closer muscles was performed.
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force were performed
muscles were implanted
muscles were examined
muscles were compared
muscles were analysed
muscles were incised
muscles were mounted
muscles were dissected
muscles was performed
muscles were cramped
muscles were detached
muscles were prepared
muscles were omitted
muscles were stimulated
muscles were injected
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