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Larval muscles were mounted in Vectashield (Vector Laboratories).
Muscles were mounted to plastic plates to keep them at their in situ resting length.
Muscles were mounted on cork supports using gum tragacanth (Sigma-Aldrich, Steinheim, Germany).
Muscles were mounted in fluorescence mounting medium (DAKO, S3023) and imaged on a Leica SP5 laser confocal microscope.
EDL and SOL muscles were mounted vertically between two Radnoti (Monrovea, CA, USA) stimulating platinum electrodes and immersed in a 20 ml bathing chamber containing the incubation medium.
After extensive washing with PBS, muscles were mounted in PBS-glycerol (1 1), covered with coverslips, and sealed with nail polish.
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Portions of each TC muscle were mounted in OCT compound (Sakura Finetech, Alphen aan den Rijn, Netherlands) and frozen in liquid nitrogen-cooled isopentane or freeze-clamped and stored at −80°C.
Freshly isolated skeletal muscle was mounted using tragacanth gum, and then quick frozen in liquid nitrogen-cooled isopentane.
A portion of the tibialis anterior muscle was mounted in OCT mounting compound and rapidly frozen in isopentane, and cooled in liquid nitrogen.
The proximal part of the right gastrocnemius muscle was mounted in an O.C.T. embedding medium (Miles, Elkhart, USA) under a microscope to orientate muscle fibers vertically and snap frozen in liquid nitrogen-cooled isopentane.
The muscles were then washed twice with MPS for 10 min and then fixed in 4% paraformaldehyde (PFA) for 20 min. After washing twice again with MPS for 10 min, the muscle was mounted in Mowiol (Calbiochem, Merck Millipore Watford, UK) and imaged using a Nikon Eclipse/Bio-Rad Nikon: Kingston-upon-Thames, UK; BioRad: Hemel Hempstead, UK.
More suggestions(15)
muscles were injected
muscles were used
muscles were implanted
muscles were examined
muscles were compared
muscles were spared
muscles were analysed
muscles were dissected
muscles were stained
muscles were harvested
muscles were frayed
muscles were cramped
muscles were detached
muscles were prepared
muscles were omitted
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