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Lanes of healthy control muscles were loaded on each gel.
After a 60 min period, during which preparations were permitted to shorten while carrying light loads, muscles were loaded to isometrically contract and stretched to the apices of their length-tension curves.
After a 60-min period, during which the preparations were permitted to shorten while carrying light loads, muscles were loaded to contract isometrically and stretched to the apices of their length-tension curves.
In the only previously described study to preserve the rotator cuff in a 2-part fracture model of the humeral head, rotator cuff muscles were loaded unphysiologically by clamping them together in one direction to a metal cylinder, which was fixed cranially in a materials-testing machine in order to analyze implant failure (Walsh et al. 2006).
Equal amounts of protein from pooled samples of either trapezius or vastus lateralis muscles were loaded on precast 10% SDS-page gels (CBS-scientific, Del Mar, CA, USA) and separated by electrophoresis (DCX-700, CBS-scientific, Del Mar, CA, USA) (running buffer: 25 mM Tris, 250 mM Glycine, 0,1%SDS).
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From a kinetic point of view, the quadriceps and hamstring muscles are loaded.
Arteries were denervated, and smooth muscle cells were loaded with calcium indicator fura-2.
For colocalization of NO and nNOS, muscle strips were loaded with the NO indicator, DAF-2DA and EFS applied as described above.
Aliquots of 10 20 μg protein from skeletal muscle or cell homogenates were loaded on 15% SDS polyacrylamide gels and tested with antibodies recognizing COXII, CI-20/NDUFB8, CII-70 kDa (Mitosciences, Eugene, Oregon, USA), COXI and Porin (Molecular Probes, Eugene, Oregon, USA) according to the supplier's recommendations.
Single muscle fibers from mdx mice were loaded with a Ca2+ indicator and subjected to stretched contractions, as described previously [18].
Gerdle and co-workers [ 9] have also found that differences between FM patients and healthy controls (HCs) in firing rate, muscle fiber conduction velocity and differential activation of the trapezius muscle are load dependent.
More suggestions(15)
muscles were implanted
muscles were examined
muscles were compared
muscles were analysed
muscles were mounted
muscles were dissected
muscles were frayed
muscles were cramped
muscles were detached
muscles were prepared
muscles were omitted
muscles were injected
muscles were used
muscles were spared
muscles were harvested
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