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Exact(28)
Satellite cells from hind limb muscles were isolated and cultured as previously described [45].
Adductor muscles were isolated and incubated in 1.8% formaldehyde for 20 minutes at 22°C.
At the indicated time points following injection, the TA muscles were isolated, frozen in liquid nitrogen and stored at −70°C until use.
Liver and skeletal muscle (quadicep, tibialis anterior and soleus muscles) were isolated and fixed in 4% paraformaldehyde and prepared as a frozen block.
Forty-eight hours after treatment muscles were isolated, fixed in 1 ml formalin buffer, and embedded in paraffin blocks following standard procedures.
The muscles were isolated, snap frozen and sectioned.
Similar(32)
Total RNA from muscles was isolated using TRIzol protocol (Invitrogen, Carlsbad, CA, USA).
Two strips of muscle were isolated from each soleus muscle as previously described [30].
Cells from muscle were isolated as previously described (26) with minor modifications (8, 9).
Heart, gastrocnemius and soleus muscle were isolated, instantly frozen in liquid nitrogen, and stored at −80 °C.
To obtain the skeletal SR fraction, the white back muscle and the leg muscle (fast twitch muscle) were isolated from Wistar rats weighing approximately 250 g.
More suggestions(15)
muscles were examined
muscles were compared
muscles were analysed
muscles were incised
muscles were mounted
muscles were dissected
muscles were cramped
muscles were detached
muscles was isolated
muscles were prepared
muscles were omitted
muscles were stimulated
muscles were injected
muscles were blotted
muscles were used
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