Sentence examples for muscles were fixed from inspiring English sources

The muscles were fixed in 4% paraformaldehyde (Sigma) in PBS overnight and paraffin embedded for hematoxylin and eosin (H&E) staining.

After sacrifice at d4, gastrocnemius muscles were fixed, dehydrated and paraffin-embedded.

Muscles were fixed in 4% PFA, sunk in 30% sucrose, frozen and sectioned in a cryostat.

For the analysis of collagen IV expression, cremaster muscles were fixed in 4% paraformaldehyde.

Stretched muscles were fixed by immersion in 4% paraformaldehyde for 2 hours at 4°C and embedded with optimal cutting temperature compound (OCT, Tissue-Tek).

Muscles were fixed and treated as described [22] except that in some cases osmication was done in the presence of 1.5% potassium ferrocyanide ("reduced osmium") for darker glycogen staining [52].

A block of proximal muscles was fixed and embedded in OCT compound as described above.

Four weeks after the transfer of BMMCs prepared from eGFP-Tg mice, a block of proximal muscles was fixed, equilibrated, and embedded in OCT compound as described above.

For immunohistochemistry, 10 micron sections of muscle were fixed in Bouin's fixative for 15 min and then oxidized in 0.3% H2O2 for 15 min. After blocking, sections were incubated with p-Smad or t-Smad 1/5/8 antibodies (1 10) overnight at 4°C.

The doses per muscle were fixed.

The disarticulated femora without muscle were fixed in formalin and stored in 10% ethanol for ex vivo specimen μCT and ex vivo DXA.