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The present study characterizes the fiber type composition of selected hindlimb muscles from two transgenic mouse lines specifically engineered to alter the amounts of circulating growth hormone (GH) and insulin-like growth factor-1 (IFG-1).

The contractile activity of muscles from two month old Vp16-Jazz transgenic mice, and wt control animals was measured.

Contractile activity of muscles from two month old Vp16-Jazz male mice and non-transgenic male littermates, was examined in vitro by physiological assessment of the muscle force of isolated diaphragm and extensor digitorum longus (EDL) preparations of both hind limbs.

Quadriceps muscles from two mdx and two Sgcg−/− D2 mice were quantified for fiber size variability (FSV) with ImageJ software.

We confirmed these data in an independent sample of 292 muscles from two wild-type controls (CS, CS/ w −) and two parkin genotypes (park /park, park / park Z3678), where the mean difference in RMP was 12.3 mV (nested ANOVA F291,1 = 168, P< 0.001).

Similar(55)

Microbiopsies were obtained from the masseter and tibialis anterior muscles from seven healthy men and seven age-matched healthy women.

The relative disposition of ryanodine receptors (RyRs) and L-type Ca2+ channels was examined in body muscles from three arthropods.

For electron microscopic studies, quadriceps muscles from three 9 10 and 12-month old knock-in and wild-type mice were fixed in 4% paraformaldehyde/0.1% glutaraldehyde/0.1 M PB.

Indirect flight muscles from three day-old flies were examined by removing heads and abdomens, dehydrating in an ethanol series, and leaving in methyl salicylate overnight.

Briefly, dissected muscles from five to six one-week-old animals (n = 20 wt and 24 mdx mice in total) were minced by a razor blade and then digested by collagenase/dispase.

Lennartsson [ 12] analyzed anterior belly of digastric muscles from five subjects and confirmed the rareness of these receptors.

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