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Despite low FPKM values, OR10AB1P was selectively expressed in striated muscles as compared to other tissues, with strongest immunoreactivity observed in skeletal muscle.
Firstly, the mRNA expression of these 4 genes was markedly lower in skeletal muscles as compared to LV myocardium (in both healthy and diseased animals).
Data from IP assays showed an increased association between SIRT-1 and PARP-1 proteins in exercised muscles as compared to intra-animal non-exercised muscles (Fig. 1H).
In the absence of acute damage, tamoxifen-treated mice did not present significant alterations or lesions in skeletal muscles, as compared with vehicle-treated controls (Supplementary Figure 14c).
Measurement of myofibre cross sectional area (CSA) demonstrated a significant increase in myofibre size in PLD1-injected muscles as compared with GFP-injected ones, as shown by a shift of the CSA distribution curve towards higher values.
The values of pressures reached at the end of this phase suggested that the action of the inspiratory rib cage muscles, as compared with that of the diaphragm, progressively decreased at higher rates with increasing level of pressure support.
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These counterintuitive findings may be due, at least in part, to the ability of mECM to attenuate muscle damage in the remaining muscle as compared to non-repaired muscle.
PPT were lower at points over the extensor carpi radialis brevis (ECRB) muscle as compared to points over the extensor digitorum communis muscle (P <.01) and over the extensor carpi ulnaris muscle (P <.001).
The muscle specific differences observed in the expression of developmental MHC with age may be influenced by the fiber type and function differences of this muscle as compared with the soleus.
Interestingly, gene expression levels mediated by PPE-MEA and PPE-HA in mouse muscle following intramuscular injection of complexes showed a reversed order: PPE-MEA/DNA complexes mediated a 1.5 2-fold higher luciferase expression in mouse muscle as compared with naked DNA injection, while PPE-HA/DNA complexes induced delayed and lowered luciferase expression than naked DNA.
This is consistent with the greater muscle loss we report in RF (extensor muscle) as compared to AT (flexor muscle).
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