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Biopsies of human cardiac muscle were obtained at the Department of Cardiothoracic Surgery, Skåne University Hospital, Lund, Sweden.
Strips of esophageal muscle were obtained from patients with achalasia and multiorgan transplant donors who served as control subjects.
Intraoperative biopsies of the rectus abdominis muscle were obtained from 18 patients with gastric adenocarcinoma (nine with CC and nine non-cachectic) and nine controls, and assayed for the expression of a set of MQC mediators.
Percutaneous skeletal muscle biopsies of the vastus lateralis muscle were obtained from R225W carriers and their matched controls using a 5 mm Bergstrom needle (Opitek) under local anaesthesia with 1% lidocaine.
Mitochondria from TA muscle were obtained as described with slight modifications.
Tendons from the pyramidal muscle were obtained from patients undergoing arthroscopic surgery.
Similar(41)
Average activity in thigh muscle was obtained from a volume of interest placed over an area of homogenous activity in the adductor muscles.
A total amount of ≈150 mg of muscle was obtained per biopsy.
DNA from breast, heart, brain, and muscle was obtained from Biochain (Hayward, CA).
Skeletal muscle was obtained from the soleus and EDL muscle of an adult female Sprague-Dawley rat from which myoblasts were isolated and cultured according to established protocols [31] [33].
Muscle was obtained by needle biopsy, with local anesthesia, from 30 subjects with normal muscles [7 young men (21 27 yr old), 8 older men (65 75 yr old), 7 young women (20 29 yr old), 8 older women (65 71 yr old)].
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