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Two strips of muscle were isolated from each soleus muscle as previously described [30].
Cells from muscle were isolated as previously described (26) with minor modifications (8, 9).
Heart, gastrocnemius and soleus muscle were isolated, instantly frozen in liquid nitrogen, and stored at −80 °C.
To obtain the skeletal SR fraction, the white back muscle and the leg muscle (fast twitch muscle) were isolated from Wistar rats weighing approximately 250 g.
Single fibres from mouse EDL or soleus muscle were isolated and cultured in order to obtain satellite cells as described recently [ 14].
One hour later, the animals were euthanized and the cerebral cortex, liver, kidney, and skeletal muscle were isolated and homogenized for the investigations.
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Sham operated limbs were surgically exposed and the PT muscle was isolated, but no tissue was removed.
To this end, extensor digitorum longus (EDL) muscle was isolated and subjected to stress tests (Table 1).
Total RNA from pectoralis muscle was isolated from a 4-month old WT, mdx, WT/mdx or mdx/mdx mice (RNeasy, QIAGEN).
The soleus muscle was isolated and cut into small pieces.
Total RNA from MSCs, bone marrow, adipose tissue and muscle was isolated by using Trizol (Qiagen).
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