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The tumours, salivary glands, thyroid (en bloc with larynx), tongue, liver, kidneys, spleen, urinary bladder, colon, upper gastrointestinal tract, skin, bone and muscle were excised and weighed, and radioactivity was measured in a gamma counter.
To evaluate PVL expression in injured tissues, infected skin and muscle were excised for PVL immunofluorescence and histologic analyses.
Samples from the liver, heart and tibialis anterior muscle were excised from adult animals.
Major thoraco-abdominal organs, the left femur and thigh muscle were excised, weighed and gamma-counted.
Organs including U87MG tumor, liver, spleen and muscle were excised, frozen and cryosectioned for histological analysis.
Pieces of foot muscle were excised and preserved in absolute ethanol.
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After euthanasia, the semitendinosus muscle was excised, separated into predominately red (RST) and white (WST) fiber type portions, and sarcoplasmic proteins were extracted.
The temporal muscle was excised, and the right MCA was exposed through a small burr hole in the temporal bone.
The entire PT muscle was excised and muscle samples were subsequently excised using an 8 mm biopsy punch through the full thickness of the muscle, following the terminal in vivo functional assessment.
To achieve a non-invasive hyperinsulinemic-normoglycemic clamp, male Wistar rats [250 300g body wt] were subcutaneously inoculated with long-acting human analogue insulin glargine (Supporting Figure S2, 42 44) or NaCl 0.9% and skeletal muscle was excised at 3, 12 and 24 hours.
Diaphragm muscle was excised for structural, biochemical and functional analyses.
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