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The proportion of centrally nucleated fibers was determined by analyzing the images and counting the number of centrally located nuclei; a total of two hundred cells per muscle were evaluated.
The deregulated genes in the IL-6 and IGF1 pathways are listed in Additional file 1: Table S1.> -wrap-foot> Gexpressionsion data for human and rat muscle were evaluated using Ingenuity Pathway Analysis.
The lower hindlimb (P0/P30) or the tibialis anterior (P30/P180) were sectioned transversally (60 μm), every fourth section was stained, and five (P0 animals) or 10 (adult animals) sections of the center of the muscle were evaluated per animal.
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Insulin-stimulated glucose uptake by skeletal muscle was evaluated ex vivo.
To quantify the immunohistochemical reaction intensity, the positive intensity MYH2 staining in 10 random fields at ×200 magnification in the masseter muscle was evaluated by computer-assisted image analysis after image transformation to grayscale.
The lean meat quality of the longissimus lumborum et thoracis muscle was evaluated by chemical analysis (moisture, protein, fat and ash), fatty acid profile, pH measurement, cooking loss, color evaluation, and sensory evaluation.
Glycogen concentration in skeletal muscle was evaluated by measuring the amount of glucose released after treatment of tissue extracts with Aspergillus niger amyloglucosidase as described [53].
For determination of immunohistological staining, a cross section of the cremaster muscle was evaluated using ×400 magnification.
The ability of torcetrapib to directly contract vascular smooth muscle was evaluated using both isolated vascular smooth muscle preparations as well as a rat perfused hindlimb preparation.
The Krickenbeck classification was used to classify anorectal malformations, and the level of the rectal ending in relation to the levator muscle was evaluated.
The structural capillary density of the skeletal muscle was evaluated with at least 12 microscopic fields randomly selected from the tissue sections.
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