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The signal-to-noise ratios (SNRs) and contrast-to-noise ratios (CNRs) of the myometrium and rectus abdominis muscle were determined on a dedicated workstation (Leonardo; Siemens Healthcare Systems, Erlangen, Germany).
Colour and pH in Semimembranosus muscle were determined, together with the analyses of the water retention capacity, loss of weight due to cooking, shearing force, and chemical composition in Longissimus dorsi muscle.
Levels of different proteins in skeletal muscle were determined by performing Western immunoblotting as described [88], [89].
Satellite-cell numbers in individual fast-twitch myofibers from the superficial region of the Gast muscle were determined based on Pax7 immune-detection (Figure 1A).
The RMS/force and MF/force relationships of each muscle were determined using linear regression.
Concentrations of free carnitine and acetyl carnitine in plasma and muscle were determined by tandem mass spectrometry using deuterated carnitine-d3 as internal standard as described recently in detail [ 44].
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The fraction of muscularis containing smooth muscle was determined by morphometric analysis of histologic cross sections.
The relative weight (g/100 g body weight) of the injured muscle (gastrocnemius muscle) was determined, which acted as an index of skeletal muscle edema in the affected limbs.
The thickness of the SUFML was microscopically measured and the density of the nonvascular smooth muscle was determined with computer-aided analysis.
Kidney injury marker-1 (KIM-1) was measured by ELISA according to the manufacturer's instructions (Rat TIM-1/KIM-1/HAVCR Immunoassay, R&D Systems, Inc .. ROS production in the injured gastrocnemius muscle was determined by measuring the concentration of TBARS.
Level of content of creatine phosphokinase (CPK) and lactate dehydrogenase (LDH) enzymes in the blood of experimental animals, as the markers of ischemic injury of the skeletal muscle, was determined by using a clinical equipment.
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power were determined
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muscle were excised
muscle were analysed
muscle were studied
muscle were purchased
muscle were used
muscle were gathered
muscle were analyzed
muscle were sutured
muscle were fixed
muscle were received
muscle were described
muscle were randomized
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