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Several pieces of the excised gastrocnemius muscle were cut from its mid-belly and post-fixed in 4% paraformadehyde.
Transverse sections (10 µm) from the midbelly region of the tibialis anterior muscle were cut on a Leitz 1720 cryostat (Leica, Wetzlar, Germany) and fixed in 4% paraformaldehyde.
PrPTSE-muscle extracts from CWD-infected cervids were prepared according to the protocol described above with modifications: 100 mg of specimens of skeletal muscle were cut into small pieces and washed twice in 1× TBS.
Serial sections from the mid-belly of the plantaris muscle were cut at 14 μm and adhered to superfrost slides.
10 μm thick cross sections from the plantaris muscle were cut in a cryostat at -23 °C and put onto glass slides.
The hearts and muscle (quadriceps muscle) were cut out from WT and KO mice and fixed in 1% glutaraldehyde in 0.2 m 4- 2-hydroxyethyl -1-piperazineethanesulfonic acid buffer.
Similar(52)
The probe-razor was not long in use before the procedure of dividing the sternomastoid muscle was dispensed with, having lost favor to a much simpler procedure in which the tendon of the muscle was cut instead.
Vacuum packed Longissimus dorsi muscle was cut into 10 × 5 × 1 cm samples and randomly dipped in one of three solutions.
The cremaster muscle was cut longitudinally with a cautery.
The harvested GM muscle was cut and minced into a coarse slurry.
No tendon or muscle is cut or detached.
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