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The length and quality of the pedicle of the gracilis muscle were confirmed preoperatively by ultrasonography and CTA (Fig. 2c).
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The importance of store-operated calcium influx in muscle is confirmed in mice lacking STIM1 which die perinatally and in patients with mutations on STIM1 or Orai1 who exhibit a myopathy exhibited by hypotonia.
The infrequency of metastases in muscle was confirmed.
Strong up-regulation of these proteins in fibrotic muscle was confirmed by immunohistochemical staining and western blot analysis.
When performing a western blot analysis on phosphoglycerate mutase 2, a higher abundance (1.21 fold increased abundance, p < 0.05 students- t-test) in the trapezius muscle was confirmed.
Absence of mitochondrial DNA depletion in muscle was confirmed in all cases by real-time quantitative PCR, as described previously (Blakeley et al., 2008).
After a positive clinical diagnosis of tightness of the upper trapezius muscle and palpable tenderness of the upper trapezius muscle was confirmed, the patient was assigned to the pain group.
The reduction of lysosomal glycogen stores (glycogenosomes) in TFEB-treated muscle was confirmed by PAS staining and by a decrease in the size of LAMP1-positive vesicles (Fig 7B and C).
MPCs in this study were defined as CD34+/Sca-1−/CD45− cells as this subpopulation has exhibited myogenic differentiation potential when isolated from both normal (28, 29) and CTX-injured (28) muscle; the myogenic potential of CD34+/Sca-1−/CD45− cells from CTX-injured muscle was confirmed in the present study.
Dystrophin presence in the EDL muscles was confirmed by IMF and by WB.
Inactivity of respiratory muscles was confirmed with the use of standard criteria [ 9].
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