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Experiments involving overexpression in the muscle were conducted at 25°C.
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Semi-quantitative analysis of calpain-3 protein in muscle was conducted as reported [ Fanin et al. 2009b].
Study Design: Bilateral laryngeal muscle recordings from the posterior cricoarytenoid, thyroarytenoid, and cricothyroid muscles were conducted in 12 ABSD patients.
Isometric exercises of the femoral muscles were conducted on the first day following the operation.
Exercises to strengthen stability and coordination of trunk and shoulder muscles were conducted at an intensity level corresponding to 50-80% of maximal muscle activity [ 25].
15 Muscle biopsies were conducted in all patients and showed inflammatory infiltrates, muscle fibre necrosis and/or expression of HLA class 1 on muscle fibres.
Muscle mechanics were conducted on the extensor digitorum longus (EDL) muscle as previously described [6].
As there was gender variation in the percentage of muscle EC found in 2 MO mice, subsequent isolations for muscle groups were conducted only in males.
Intraoral and extraoral muscle palpation were conducted according to RDC guidelines.
Electromyography and muscle biopsy were conducted under general anaesthesia.
In vitro studies, including primary aorta endothelial or smooth muscle cells, were conducted to evaluate whether arsenic induces cellular mechanisms relevant to atherogenesis such as endothelial dysfunction, lipid oxidation, and smooth muscle cell proliferation.
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