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Perfusion ratio between gene transduced muscle and intact muscle was quantified with DataPro 2.13 software (Noesis) using the maximum signal intensities.
The vascular area in the ear, skin or muscle was quantified as a PECAM-1/CD31-positive area from ten ×10 micrographs, using Image J software (http://rsb.info.nih.gov), as described elsewhere [37].
The perfusion function of the muscle was quantified by a proposed model to obtain analytic results.
LDMA, a marker of lipid-rich skeletal muscle, was quantified using CT and cannot distinguish between extra- and intramyo-cellular lipids.
Reinnervation of the gastrocnemius muscle was quantified by taking a complete mosaic picture of two whole sections of muscle per animal at ×20 magnification with a Z-stack through the entire thickness of the muscle on the confocal Zeiss LSM 700 laser scanning microscope.
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The asymmetry between the activity of the left and right jaw muscles was quantified by the Asymmetry Index (As, unit %).
The asymmetry between the activity of the left and the right jaw muscles was quantified by the Asymmetry Index (As).
Muscles were identified based on their anatomic features, and the structure of those specific muscles was quantified based on pre-established thresholds of skeletal muscle tissue (−29 to +150 Hounsfield units; Mitsiopoulos et al, 1998).
To evaluate the fibrosis in each muscle, the amount of intermyofibre connective tissue formed in selected muscles was quantified in situ by utilizing the specific binding of wheat-germ agglutinin (WGA) to N-acetylglucosamine and sialic acid residues in proteoglycans, polysaccharides and glycoproteins in the extracellular matrix (Dunn et al. 1982; Kostrominova 2011).
The resulting contractions of the adductor pollicis muscles were quantified by an acceleromyographic monitor.
The ability to recruit these muscles is quantified by assigning 1 of 6 defined grades.
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