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In the mid-1960s, Paul Fatt worked with his then wife Gertrude Falk on the membrane system of skeletal muscle using the electrical impedance methods developed by K.S. Cole to understand how skeletal muscle had a membrane capacitance that far exceeded the value predicted from muscle fiber size.
The EUL domain of all putative EUL sequences from the monocot and dicot species under study was extracted and aligned with MUSCLE using the default settings (Edgar 2004).
Sequences corresponding to the different EUL domains of the OsEULs were extracted and aligned with MUSCLE using the default settings (Edgar 2004).
Total lipids were extracted from frozen muscle using the method of Folch et al. [23].
Total RNA were isolated from quadriceps muscle using the Trizol method (invitrogen).
Total RNA was isolated from ∼50 mg of quadriceps muscle using the mirVana™ miRNA isolation kit (Ambion Inc., Austin, TX) according to the manufacturer's instructions.
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Dr. Levine said the youngest patient he had treated was 12. Dr. Young, who overworked his core muscles using the Wii Fit, is 32.
We established a method for fabricating multifilament muscles using the thin McKibben muscle that we previously developed.
Total RNA was extracted separately from EDL and soleus muscles using the Trizol protocol (Invitrogen).
We applied muscle forces over the origins of the temporalis, masseter and pterygoideus (internal+external) muscles using the tangential-plus-normal traction model in the program BoneLoad [15].
(E ) Live image of Spin-GFP expressed in muscles using the muscle-specific BG57-Gal4 driver.
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