Muscle tissue samples have been collected from 156 red deer to provide a source of DNA for this project.
The body lengths (BL) of the specimens were measured on site, and muscle tissue samples were collected and dried prior to preservation.
Total genomic DNA was extracted from the muscle tissue samples using the Wizard® Genomic DNA Purification Kit (Promega) according to the manufacturer's protocol.
Nearly 15 years later, Hollidge-Horvat and colleagues used human muscle tissue samples obtained at different steady-state exercise intensities (i.e. 30, 60 and 75%% VO2max) to investigate the mechanisms responsible for the increased La− efflux by then commonly observed after NaHCO3 ingestion [57].
Fin clips or muscle tissue samples were used to extract DNA from all samples.
Importantly, qRT-PCR did not detect viral RNA in any internal organs or muscle tissue samples.
For histological analysis of muscle tissue, samples were fresh dissected and frozen in melting isopentane in dry ice.
GD1b was used as internal standard, because endogenous GD1b was undetectable in muscle tissue samples (data not shown).
Collected lobsters were dissected to obtain abdominal muscle tissue samples in the field, which were stored on ice in glass scintillation vials until frozen in the laboratory.
Frozen muscle tissue samples from wt and mdx mice were homogenized in lysis buffer (2% SDS, 5 mM EDTA) supplemented with proteinase inhibitors, boiled for 5 minutes and centrifuged at 12,000 g for 10 min [48].
Briefly, frozen skeletal muscle tissue samples (25 35 mg) were hand homogenized in 25 µl of phosphate buffer (50 mM Kpi, 5 mM EDTA, 0.5 mM DTT, 1.15%KCl (w/v)) per milligram of tissue.
