Genomic DNA was isolated from muscle tissue of the six specimens using FastDNA kit® (Q-Biogene).
Total genomic DNA was extracted from small amounts of tissue (1 musclescle tissue or whole legs for small specimens) using the Chelex dry release [166] or QIAGEN DNeasy tissue extraction kits (QIAGEN) for older or less well preserved samples.
In addition, adult donor muscle specimens were used when indicated.
Genomic DNA was extracted from liver or muscle tissues of T. alpinus T. amoenus T. ruficaudus, and T. striatus specimens using Qiagen DNeasy Blood & Tissue kits.
Total genomic DNA was extracted from the muscle of a specimen using the OMEGA Genomic DNA Extraction Kit.
For diagnostic purposes deltoid, quadriceps, and biceps muscle specimens were collected using institutionally approved protocols and after obtaining informed consent (Table 1).
Total genomic DNA was extracted from muscle tissue of each specimen using a DNeasy Tissue Kit (QIAGEN) according to the manufacturer's protocol.
For each species, total genomic DNA was extracted from the thorax muscle of a single specimen using a DNeasy Tissue Kit (Qiagen) according to the manufacturer's protocols.
Specimens used in this study.
All muscle tissues tested in our study were prepared under sterile laboratory conditions from inner parts of muscle specimens harvested at necropsy using separate sets of instruments and separate table coverings.
