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Experiments are run using both synthetic data and muscle specimen data.
Human cadaveric muscle specimen data provides the anatomical detail needed for in-depth understanding of muscle and accurate parameter estimation.
In the second experiment, the muscle specimen was prepared through a modification of previously described methods [15, 16].
Briefly, muscle specimen was disintegrated, using scissors, in the isolation solution and treated with 1 ml of 0.2 mg/ml protease (Sigma P-4789) for 2 min followed by homogenization (Potter Elvehjem homogenizer) and washing with isolation solution.
An additional muscle specimen was placed in PBS and used for the establishment of primary myoblast culture for some individuals (Table 1).
In comparison to peripherally located nuclei in the representative normal TA muscle specimen (Fig. 5C), abundant centralized nuclei were present upon infusion with heterogeneous cells or B6 clones (Fig. 5A and 5B), suggesting neomyofiber formation and muscle regeneration.
Similar(44)
Methods: Fetal lambs underwent harvest of skeletal muscle specimens.
Another investigative tool is the muscle biopsy, which provides muscle specimens for pathological diagnosis and biochemical analysis.
To define threshold times for ryanodine contracture testing (RCT) using skeletal muscle specimens from malignant hyperthermia-susceptible (MHS) and control individuals.
Twelve OCI muscle specimens were excised, 100% of which emitted grossly visible soft tissue tracts that inserted into the posterolateral aspect of the cervical dura.
Oligonucleotide fragments of length between 108 and 577 base pairs were amplified from all groups of alcohol-fixed skeletal muscle specimens in amounts comparative to the unfixed muscle tissue.
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