Sentence examples for muscle sections using from inspiring English sources
This decreased protein expression was also reflected in the low GFP fluorescence intensity observed in CAG200 muscle sections using a fluorescence microscope (Fig. 2D).
The cross-sectional areas of the muscle fibers of from animals of each genotype were calculated from SDH-stained musectionstions using three sections from the belly of TA muscles from each mouse (n = 3).
For immunofluorescence after cell transplantation, tibialis anterior muscles were excised and frozen prior to the collection of transverse muscle sections using a Leica CM 3050S cryostat.
Muscle fiber cross-sectional area of SDH-positive oxidative fibers and SDH-negative glycolytic fibers was determined from digitized muscle sections, using ImageJ software (http://imagej.nih.gov).
The presence of rimmed vacuoles and protein aggregates was assessed on immunohistochemical staining of muscle sections using anti-SQSTM1 antibody (Fig. S5A).
Results obtained from manual analysis of whole muscle sections of TA muscle (n = 4) of 6 month old mdx mice were compared to results received form automatic analysis of the same muscle sections using MyoScan.
Immunofluorescence analysis of AAV-transduced mKO muscle cross-sections using novel polyclonal antibodies (discussed later) demonstrated that overexpressed myotubularin is present in most myofibers (Fig. 2A) and located throughout the cytoplasm with a clear reinforcement in the sarcolemmal region.
Muscle was sectioned into six‐micron sections using a cryostat (Bright Instruments Co., Huntingdon, UK) and used staining protocols described in the Supplemental Experimental Procedures.
Serial muscle sections were used to estimate and compare proportions, cross-sectional areas (CSAs), and %CSAs of Type 1, Type 2, and hybrid fibers between species.
Cardiac muscle sections were used as positive controls, while absence of lectin served as negative control.
Skeletal muscles were sectioned using a cryostat, fixed with ice-cold acetone at −20°C for 10 min, and stained with laminin to visualize the myofibers.
