Muscle samples were homogenized with a Precellys 24 (Bertin Technologies).
Semi-membranosus muscle samples were removed 24 h post-mortem and lipids extracted with chloroform methanol.
Muscle samples were blotted and immediately frozen in liquid N2 before being homogenized in ice-cold lysis buffer containing protease and phosphatase inhibitors48.
Blood and muscle samples were taken before, directly after, and 2 h after the 70% of VO2max bicycle test, before as well as after 12 weeks of training.
Dorsal skin and femoral skeletal muscle samples were obtained and examined by CD117, CD34, semi-thin and ultrathin sections examination.
Muscle samples were obtained from the vastus lateralis of one limb.
Skeletal muscle samples were subjected to immunoblot analysis as described previously [47].
Skin and muscle samples were used for immunohistochemical analyses and examined by light and electron microscopy.
Muscle samples were collected with the informed consent of the participants.
Muscle samples were fixed in RNAlater storage solution (Ambion Inc., Woodlands, TX, USA) immediately after collection.
Blood and muscle samples were collected before and at 4, 24, 72, and 120 hours post intervention.
