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In this paper the experimental and modelling results of compressive loading of freshly slaughtered porcine muscle samples using a drop-tower testing rig are reported.
We compared CpG methylation changes with age across 283 human blood, brain, kidney, and skeletal muscle samples using methylation arrays to identify tissue-specific age effects.
RNA was isolated from homogenized muscle samples using the TRIzol/RNeasy method.
Total RNA was isolated from muscle samples using TRIzol (Invitrogen, Stockholm, Sweden) and quantified using a Nano-drop spectrophotometer.
RNA was isolated from homogenized muscle samples using TRIzol method and a Total RNA Kit as previously decribed [27].
By default, ComparativeMarkerSelection compared the mean difference in gene expressions between weight bearing and unloaded muscle samples using two-way parametric t-tests and computed false discovery rate for each probe set using the Q-value [42].
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Protein lysates (20 µg of total protein) from the same skeletal muscle samples used for the microarray study were separated on 10% BIS-TRIS gels and proteins were transferred to nitrocellulose membranes (all from Invitrogen).
All muscle samples used in this were collected as described previously [ 5].
Table 1 presents summary statistics for all muscle samples used in this study.
This discrepancy may be due to experimental variations or slight biological differences between the batches of muscle samples used for microarray and RT-qPCR validation.
Alternatively, FABP4 is not detected as differentially expressed because all intramuscular fat was removed from the muscle samples used in this study (see details in the methods section).
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