Sentence examples for muscle samples according from inspiring English sources
Two specific target amplifications (STA) were performed on cDNA muscle samples according to the manufacturer's recommendations.
The results were shown in Tables 1 and 2. The rabbit ileum was dissected out and cut into 2 cm segment for preparing isolated intestinal smooth muscle sample according to previous description [ 30].
Messenger RNA from liver or gastrocnemius muscle was isolated from fresh snap frozen tissue samples according to Qiagen protocols for RNeasy and RNeasy Fibrous Tissue kits.
Fatty acid composition in muscle samples was measured according to methodology previously described [17], [19].
The collection of muscle samples were performed according to the methods described by Schmidt et al. [ 24], whereby the extracted pieces were stored in a polystyrene box with ice and protected by a gauze pad.
Total RNA was extracted from GA muscle samples with TRIZOL reagent (Invitrogen), according to the manufacturer's instructions.
Total RNA was extracted from LD, SS and ST muscle samples using TRIZOL (Invitrogen, Paisley, UK), according to the manufacturer's instructions, and then treated with DNase (Promega, Southampton, UK).
Total RNA was isolated from breast muscle samples using Trizol reagent (Invitrogen, USA) according to the manufacturer's instructions and dissolved in RNase-free water at a final 2.0 μg/μl concentration.
Muscle samples were dissociated using a Medimachine (BD Biosciences) according to the manufacturer's instructions.
DNA was extracted from the muscle samples using the DNeasy Blood & Tissue Kit (Qiagen GmbH, Hilden, Germany) according to the manufacturer's instructions.
