Sentence examples for muscle sample was from inspiring English sources
Briefly, approximately 25 mg of each muscle sample was homogenized in a total of 1.0 mL of TRIzol Reagent (Invitrogen Corporation, Canada) using a rotary homogenizer.
Briefly, each muscle sample was homogenized in a total of 1.0 ml of TRIzol Reagent (Invitrogen Corporation, Canada) using a rotary homogenizer.
The muscle sample was immediately frozen (in 5 10 s) in liquid nitrogen and stored at −80°C for subsequent metabolite assay, after being freed from visible fat and connective tissue.
Total RNA (750 ng from each muscle sample) was converted into cDNA in 20 µl using the OmniScript reverse transcriptase (Qiagen, Valencia, CA, USA) according to the manufacturer's instructions.
The arctic fox muscle sample was collected in central Siberia (Additional file 1).
The muscle sample was then loaded on 0.75 mm-thick polyacrylamide gels for protein separation.
According to our experience, the average reconstruction speed for the whole muscle sample is ∼0.5 mm per hour [7].
Fatty acid composition in muscle samples was measured according to methodology previously described [17], [19].
Absorbance was normalized to the sample weight and ratio between gene transferred and intact muscle samples was calculated.
RNA from Longissimus thoracis muscle samples was hybridised on the chips.
Muscle samples was sectioned and transferred to impregnated slides in ice-cold acetone prior staining.
