The non-invasive and non-ionizing MRS is considered gold standard in muscle lipid quantification [ 10] and may in the future replace liver biopsies as the gold standard in the quantification of liver fat, although it is not providing information regarding histological alterations [ 10– 10].
Confirming the distinct effects of hyperactive Wnt/β-catenin on slow versus fast muscle fibers, quantification by RT-qPCR of myosin heavy chain specific for slow or fast twitch muscle fibers revealed an increase in fast muscle myosin at 54 hpf (Fig. 2B).
The results of muscle uptake quantification are listed in Table 1.
Detailed procedures of plaque surface, macrophage and smooth muscle cell quantification, as well as plaque quality assessment are described elsewhere.
Quantitative MRI methods such as T2 relaxation time measurements, muscle fat quantification using the 3-point Dixon technique, magnetic resonance spectroscopy and perfusion imaging might be helpful to further analyse the degree of pathological changes in the striated muscles [ 32– 32].
To examine muscle remodeling, quantification scores for inflammation and regeneration were converted to a rank-order scale, the data was pooled, and a Wilcoxon rank-sum test compared differences between treated and untreated mice.
Our near feature plan is to include the ocular VEMPs recording in our routine diagnostic tests ensuring that the adaptation of the muscle activity quantification methods we use is properly utilized.
Plasmids were electrotransferred into tibialis anterior mouse muscle and quantification of bioluminescence was performed by ex vivo measurement with a luminometer as well as non-invasive in vivo imaging with a CCD camera.
Recently introduced advanced and quantitative MRI methods including T2 relaxation time measurements, muscle fat quantification using the 3-point Dixon technique, magnetic resonance spectroscopy and perfusion imaging are quite promising, but also challenging in terms of the quantification and further specification of the disease process and disease monitoring.
The number of LacZ-positive cells was higher in ctx-treated muscles, but LacZ fibers were clearly detected also in untreated muscles (a quantification is reported in Figure 3b).
