Sentence examples for muscle plating medium from inspiring English sources

Each single fiber was subsequently transferred in another 10 cm plate containing 10 ml of muscle plating medium (2° dilution).

In each preparation, under phase contrast microscope, 50 single fibers were carefully sucked up through a 100 µl pipette and transferred in a 10 cm-plate containing 10 ml of muscle plating medium (1° dilution).

Following digestion, the muscle was transferred in plating medium (DMEM low-glucose, 10% HS, 1% penicillin-streptomycin, 0.5% chicken embryo extract, GIBCO-Invitrogen) and gently triturated with a wide-bore pipette to release single myofibers.

To examine any possible effects of hESC secreted pro-regenerative factors on activation, quiescent satellite cells were isolated from uninjured mouse muscle and plated in a low-mitogen plating medium, with an equal volume of hESC conditioned Opti-MEM or FGFs added in Opti-MEM.

Next day, cells were treated as indicated in plating medium.

Cells were washed and the medium was replaced with plating medium containing the indicated treatments.

After 18 24 hrs, medium was replaced with plating medium containing the indicated concentration of curcumin.

Single fibres from EDL muscle of 3 4 month-old mice were plated in each well (∼10 fibres per well) of a Matrigel-coated six-well plate in plating medium (10% horse serum in DMEM, 0.5% chick embryo extract, and 1× P/S/ l-Glutamine) at 37°C, 5% CO2.

All media including media stock, glial plating medium, neuronal plating medium, mixed culture plating medium and maintenance medium have been fully described (Jackman et al., 2010b).

Cells were plated 400 µl/well in glial plating medium (two hemispheres/10 ml/plate; Falcon Primaria 24-well plates; BD Biosciences, Lincoln Park, NJ).

On the second day after plating, medium was replaced.