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The airway internal perimeter (Pi), wall area (WA), the area of bronchial smooth muscle (S) and the number of bronchial smooth muscle nucleus (N) were measured.
(A ) Mitochondrial morphology based on anti-Complex V antibody staining (Complex V) in larval muscles (Zoom in view around muscle nucleus).
We provide evidence that Hts promotes the phosphorylation, and thus delocalization, of Dlg at the postsynaptic membrane by regulating the re-distribution of par-1 and camkII transcripts from the muscle nucleus to the cytoplasm.
In the next step, immunofluorescence staining of quadriceps muscles revealed significantly higher accumulation and co-localization of NFkB p65 subunit in the muscle nucleus of treated and untreated dy 2J /dy 2J mice, compared with WT mice (untreated dy 2J /dy 2J : 20.57±1.25% versus WT: 0.166±0.166% P<0.00001 and treated dy 2J /dy 2J : 20.71±2.59% versus WT: 0.66±0.49% P<0.00001).
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Eventually myotubes can grow to include hundreds of muscle nuclei, and they then undergo terminal differentiation to become mature muscle fibres.
This integration partly occurs in the vestibular nuclei, the source of secondary neurons destined for the extraocular muscle nuclei of both sides.
In muscle nuclei these speckles cannot be detected.
In addition, these nuclei showed phalloidin staining fibers within the nucleus (example shown in Fig. 5B); these fibers were never observed in muscle nuclei of wild type larvae.
First, we observed that NF-YA is expressed in the nuclei of the mdx muscle fibers, whereas it is absent in wt muscle nuclei (Figure 5C).
Confocal microscopy showed abnormal shaped nuclei, as evidenced by irregular anti-lamin Dm0 staining in 25% of the muscle nuclei (Fig. 5A).
Using body wall muscle nuclei as a 2 n DNA standard, C values of intestinal nuclei were estimated by their DAPI-based densitometric quantifications [57], [58].
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