Almost all the ZHTc6-MyoD cells were induced into muscle lineage after removal of tetracycline.
The tumor was suspected to have been originated from myoepithelial cells because of the expression of smooth muscle lineage with Calponin1.
In contrast, two markers of early smooth muscle lineage progression, myocardin and SM-α-actin, were significantly elevated in the "stripe" pattern relative to the "loop" pattern.
Therefore, it is possible to obtain a large quantity of ZHTc6-MyoD cells in the undifferentiated state that maintain the potential to differentiate only into muscle lineage.
Interestingly, uniaxial cell alignment in the ST pattern seemed to increase the expression of SM22α and smooth muscle α-actin, suggesting an early smooth muscle lineage progression.
Our experiments could show that the missing vasculogenic potential of pbEPCs is not rescued by coimplantation of SMCs derived from MSCs predifferentiated along the vascular smooth muscle lineage.
To design scaffolds which specifically induce MSC smooth muscle lineage progression requires a deeper understanding of the relative influence of various microenvironmental signals on myogenesis.
describe an unexpected role for the histone methyltransferases MLL3 and MLL4 in the repression of tissue-specific promoters, a function that prevents precocious cell differentiation in the skeletal muscle lineage.
With bioreactor cultivation, progenitor cells differentiated toward smooth muscle lineage characterized by the expression of smooth muscle (SM -specific markerSM -specificcle alpha actin (Smarkersin) and smooth muscle myosin healphahactinSM-α-actin
In immunohistochemical findings, the tumor was suspected to be originated in myoepithelial cells because of the expression of smooth muscle lineage including α-smooth muscle actin and Calponin1 and highly malignant characteristics showing MIB-1 and p53 highly positive with angiogenesis.
These experiments indicate that dedifferentiating muscle remains restricted to the muscle lineage [16].
