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Experiments were conducted on both the right and left EDL muscle for each mouse.
The percentage muscle for each section was calculated and the overall average taken for both groups.
A multiple sequence alignment was produced using MUSCLE for each protein (Edgar 2004), and divergent or ambiguous positions were removed.
Intramuscular fatty acid composition of semitendinous muscle for each feeding system is reported in Table 1 and Figure 2. Different subscripts differ with at least P < 0.05.
The mean fold changes of the expression of these genes in exercised vs. rested muscle for each sex and time specific condition are presented in supplementary materials (Additional file 6, Table S6).
Emission signals at 615 nm from the labelled sections excited at 530 585 nm were captured as 10 23 or 3 6 images for respectively one whole section of the left costal part of diaphragm or left EDL muscle for each mouse using a ×10 objective as described in the previous paragraph.
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A thin layer of plates (10−4mm) was applied over the entire area of muscle origin for each muscle.
Muscle scores for each muscle section were considered separately.
Finally, spindle density was evaluated as the spindle number per gram of wet muscle tissue for each muscle sample.
We designed this interface to make a cursor move on a two-dimensional screen and to click using only three muscles for each subject.
All brown staining capillaries were counted for each of 5 to 6 sections for both the left and right anterior tibialis muscles for each experiment and then were averaged.
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CEO of Professional Science Editing for Scientists @ prosciediting.com